E-Abstract

JACC

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TCTAP A-072

Potential Genes and Pathways Related to the Foam Cell Formation Identified via Microarray Gene Expression Dataset Re-Analysis

By Jing Xu

Presenter

Jing Xu

Authors

Jing Xu1

Affiliation

Fuwai Hospital, China1
View Study Report
TCTAP A-072
Basic Science, Animal Models and Preclinical Studies

Potential Genes and Pathways Related to the Foam Cell Formation Identified via Microarray Gene Expression Dataset Re-Analysis

Jing Xu1

Fuwai Hospital, China1

Background

Atherosclerosis represents the leading abnormal condition of cardiovascular diseases. Foam cells play a crucial role in atherosclerotic lesion development, and macrophages and vascular smooth muscle cells (VSMCs) appear to be the majority of the cellular origins of foam cells formation. 

Methods

Here we conducted an integrated microarray-based analysis based on GSE54666 and GSE68021 which contain samples of human macrophages and VSMCs incubated with oxidized low-density lipoproteins(ox-LDL). The differentially expressed genes (DEGs) in each dataset were investigated via the limma package in R. Geno ontology (GO) and pathway enrichment were performed via the ClueGO, CluePedia, and DAVID database. The convergent DEGs in the two cell types were obtained, and the protein interactions and transcriptional factors network were analyzed using STRING andTRRUST v2 database. 

Results

For monocyte-derived macrophages, under the treatment of ox-LDL, a total of 504 DEGs were identified, and the top 5up-regulated DEGs were AKR1C3, PLIN2, PDK4, TMEM158,and FABP4, while the top 5 down-regulated DEGs were LDLR, F13A1,SQLE, INSIG1, CD93. For VSMCs, a total of 4400 DEGs were identified, and the top 5 up-regulated DEGs were RGS7BP, RFTN2, CALCRL,OCLN, HSD17B6, and the top 5 down-regulated DEGs were ACTG2,BEX1, IFI30, TGM2, TFPI2 in response to ox-LDLtreatment. 

Conclusion

Our study has the merit of providing a comprehensive landscape of the transcriptional regulations in macrophages and VSMCs under the ox-LDL treatment from a bioinformatics respective, as well as suggesting several multi-cellular biomarkers and candidate pathways that might participate in the initial atherogenic events, which holds considerable promise to emerge capability to ascertain corresponding mechanisms and offer projections into developing new approaches for potential therapeutic modulation and cardiological practice.

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