Basic Science, Animal Models and Preclinical Studies
5-Oxymethyluracil Stimulate Neoangiogenesis in Postinfarction Cardiosclerosis Model in Rabbits
Bogdan Oleynik1, Vladimir V. Plechev2, Gulnara Bayburina1, Roman Risberg2, Igor V. Buzaev1
Bashkir State Medical University, Russian Federation1, Republican Cardiology Center, Russian Federation2
To study the effectiveness of pyrimidine - 5-oxymethyluracil as a neoangiogenesis stimulator by determining the density of the vascular network and structural changes of the left ventricle in a postinfarction cardiosclerosis model in rabbits.
The study was conducted on 53 male Chinchilla rabbits weighing 2.0-2.5 kg, in which a postinfarction myocardium model was reproduced by ligation of the anterior descending artery at the border of the initial and middle parts. 4 weeks after the operation, all animals were divided into 2 groups as follows: the experimental group (27 animals) received orally 5-oxymethyluracil drug at a dose of 25 mg/1 kg of live weight 3 times a day for 14 days, the control group also consisted of 26 rabbits. Animals were removed from the experiment by Arduan overdosing 2 weeks after the end of the drug administration.Histological sections were stained with hematoxylin-eosin and pyrofuxin according to Van Gieson. The borderline zone with the scar was evaluated. In each preparation, in five consecutive fields of view with an increase of x400, the number of all vessels was counted, that is arterioles, capillaries, venules, veins and sinuses. Dilation of the left ventricle was assessed by heart sections. The photographed slices were subjected to the Adobe Photoshop computer program. Dilation of the left ventricle was calculated as the ratio of the area of the left ventricular lumen to the area of the myocardium of both ventricles. The results of the left ventricular dilation index were calculated from three sections and the average values were represented.
The density of the vascular network and the left ventricular dilation index in groups of animals with and without 5-oxymethyluracil therapy 2 weeks after the end of the drug administration, M± σ is shown in Table 1. (p - statistical significance of the differences between the control and main groups was calculated using single-factor analysis of ANOVA variance).
| Indicators || Group || р |
| Control, n=26 || Main, n=27 |
| Number of vessels in one field of view (х400) || 4,92±1,26 || 9,40±1,19 || <0,0001 |
| LV dilation index, rel. units || 0,23±0,07 || 0,19±0,05 || 0,0055 |
The representative of pyrimidines - 5-oxymethyluracil leads to a significant increase in the density of the vascular network in the zone on the border of postinfarction cardiosclerosis by 91% and prevents the remodeling of the left ventricle in the postinfarction cardiosclerosis model in rabbits (the left ventricular dilation index in the main group is 17.2% lower than in the control group).